Journal: Cell reports

Article Title: Aedes aegypti sialokinin facilitates mosquito blood feeding and modulates host immunity and vascular biology

doi: 10.1016/j.celrep.2022.110648

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human Primary Dermal Microvascular Endothelial Cells (HDMVECn, ATCC®, Cat# PCS-110-010) were grown in Vascular Cell Basal Medium (ATCC®) supplemented with Microvascular Endothelial Cell Growth Kit-BBE (ATCC®) on 75 cm 2 culture flasks according to the manufacturer’s instructions.

Techniques: Purification, Blocking Assay, Virus, Titration, Recombinant, Lysis, DNA Purification, Staining, Luminex, Mass Spectrometry, Software

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD39 , REA739 , 50 , 130-110-650 , PE , Miltenyi Biotec.

Techniques: Imaging

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Cellular neighborhood analysis of PD1 high/low T cells in the tumor margin and core. (A–D) Topology of PD1 high (left) and PD1 low (right) T cells and their cellular neighborhood within a 5 µm range. (A, B) represent tumor margin and (C, D) show tumor core areas. Cell types showing different distribution patterns around PD1 high and PD1 low T cells (mDCs, M1-like M, M2-like M, MDSCs, Fibroblasts, vessels, tumor cells) are highlighted by arrowheads. (E, F) Quantification of cells in a 5 µm range around of PD1 high/low T cells for tumor margin and tumor core, (E) represents immune cells and (F) stroma/tumor cells. (G) Violin plots for expression levels of eight immune-modulating markers (CD112, CD155, CD276, CD39, CD73, IDO, PD-L1, and VISTA) for the most important immune and tumor cells around PD1 high/low T cells in the tumor core area. Violin plots for tumor margin are shown in Supplementary Figure S5E . Depicted markers and annotated cell types as indicated by the color code. ROI sizes: Tumor margin (ROI15) and tumor core (ROI16): 975 x 769 µm.

Article Snippet: CD39 , REA739 , 50 , 130-110-650 , PE , Miltenyi Biotec.

Techniques: Expressing

Journal: Nature biomedical engineering

Article Title: Robotic fluidic coupling and interrogation of multiple vascularized organ chips.

doi: 10.1038/s41551-019-0497-x

Figure Lengend Snippet: Fig. 3 | Linking scheme of eight organ HuBoCs. a, Human organs for the HuBoC system, each with a representative organ-chip photograph and sectional schematic. BBB: the lower channel contains brain microvascular endothelial cells, while the upper channel contains brain pericytes and cortical astrocytes. Brain: the chip contains differentiated human primary neural stem cells forming networks of neurons and astrocytes. Lung: the lower channel contains HUVECs, while the upper channel contains lung epithelial cells. Skin: the lower channel contains dermal microvascular endothelial cells, while the upper channel contains keratinocytes and dermal fibroblasts. Kidney: the lower channel contains kidney-derived endothelial cells, while the upper channel contains proximal tubule epithelial cells. Gut: the lower channel contains HUVECs, while the upper channel contains villi-like structures of gut epithelial cells. Liver: the lower channel contains liver sinusoidal endothelial cells, while the upper channel contains hepatocytes. Heart: the lower channel contains HUVECs, while the upper channel contains stem cell-derived cardiomyocytes. Scale bar, 5 mm. b, A total of eight vital organs—gut, liver, heart, kidney, lung, heart, brain, BBB and skin—were joined through vascular endothelial channels to create the body on chips. The system enables multiple sampling points and a variety of linking possibilities.

Article Snippet: Human dermal microvascular endothelial cells (HDMVECs; ATCC, PCS-110–010) were cultured in vascular Cell Basal Medium (ATCC, PCS-100–030) supplemented with an endothelial cell growth kit-VEGF (ATCC, PCS-100–041).

Techniques: Derivative Assay, Sampling

Journal: Emerging Microbes & Infections

Article Title: Cultivation of the causative agent of human neoehrlichiosis from clinical isolates identifies vascular endothelium as a target of infection

doi: 10.1080/22221751.2019.1584017

Figure Lengend Snippet: Clinical data of patients whose blood samples were included in the study.

Article Snippet: SE24 , 54 , M , Granulomatosis with polyangiitis , Rituximab Chemotherapy IL-1R inhibitor Corticosteroids , 1.5 years , Severe fatigue, skin rash, general myalgia, cough , DVT lower extremity , Yes , Whole blood and plasma/buffy coat , IRE/CTVM20 ATCC PCS-110-010 ATCC PCS-100-022 , Tick cells: 9 weeks Endothelial cells: 15 days , NA , This study.

Techniques: Infection, Clinical Proteomics